Interactions Between Fibroblast Cells and Laser Beam Welded AISI 2205 Duplex Stainless Steel

  • Ceyhun KÖSE Faculty of Natural Sciences and Engineering, Department of Mechanical Engineering, Gaziosmanpaşa University, Tokat, Turkey
  • Ramazan KAÇAR Faculty of Technology, Department of Manufacturing Engineering, Karabük University, Karabük, Turkey
  • Aslı Pınar ZORBA Department of Bioengineering Cell Culture and Tissue Engineering, Yıldız Technical University, Istanbul, Turkey Vocational School of Health Services, Bilgi University, Istanbul, Turkey
  • Melahat BAĞIROVA Department of Bioengineering Cell Culture and Tissue Engineering, Yıldız Technical University, Istanbul, Turkey
  • Emrah Şefik ABAMOR Department of Bioengineering Cell Culture and Tissue Engineering, Yıldız Technical University, Istanbul, Turkey
  • Adil M. ALLAHVERDİYEV Department of Bioengineering Cell Culture and Tissue Engineering, Yıldız Technical University, Istanbul, Turkey
Keywords: laser beam welding, duplex stainless steel, L929 fibroblast cell, viability, microstructure

Abstract

Because of their high mechanical strength, excellent corrosion resistance and good weldability, duplex stainless steels are mostly used in industries such as oil, chemistry, petrochemistry, food and occasionally used in medical industry. These properties have enabled us to use duplex stainless steels in biomedical applications recently. Accordingly, duplex stainless steel material can be highly important to examine the toxic effect on the cells. In this study, the effect of the AISI 2205 duplex stainless steels which are joined by CO2 laser beam welding on viability of L929 fibroblast cells has been studied in vitro for the first time. For this aim, the cells were kept in DMEM/F-12 (Thermofisher Scientific 31331-028) medium for 7 days. The viability study was experimentally studied using the MTT (Thiazolyl Blue Tetrazolium Bromide) method for 7 days. The cell viability of the laser beam welded sample has been detected to be higher than that of the base metal and the control based on 7th day data. According to the obtained results, it was revealed that laser beam welded and base metal AISI 2205 duplex stainless steel has been found suitable to study for biomedical applications.

DOI: http://dx.doi.org/10.5755/j01.ms.24.2.18006

Published
2018-05-22
Section
METALS, ALLOYS, COATINGS