Synthesis, Characterization and Blood Based Toxic Effects of Superparamagnetic Nanoparticles

  • Muhammad Javid ARSHAD Department of Basic Sciences.University of Engineering & Technology, Taxila
  • Saddique AMIR Department of Basic Sciences (Physics), University of Engineering and Technology, Taxila
  • Mehmood ZAHID Department of Software Engineering, University of Engineering and Technology, Taxila, email;
  • Amin NIAMA Department of Physics, COMSATS Institute of Information Technology, Lahore, 22060
  • Husain FAYYAZ Department of Physics, Bahudin Zakariya University, Multan, Pakistan, 60800
  • Saeed Ahmad BUZDAR Department of Physics, The Islamia University of Bahawalpur, Pakistan, 63100
  • Muhammad Anjum NAEEM Department of Physics, The Islamia University of Bahawalpur, Pakistan, 63100
  • Muhammad IMRAN Department of Physics, Government College University, Faisalabad, Pakistan, 3800
Keywords: iron oxides nanoparticles, complete blood count, hemoglobin, erythrocytes, leucocytes

Abstract

The objective of this study is to the assess toxicity of superparamagnetic nanoparticles within normal and diabetic human blood groups using complete blood count (CBC). For this purpose, iron oxide nanoparticles (IONPs) were synthesized by co-precipitation method. Samples were characterized by XRD, SEM, EDS, VSM and CBC. XRD confirmed the cubic structure of Fe3O4 with Miller indices (2 0 0), (2 2 0), (3 1 1), (4 0 0), (4 4 0) and average size of magnetic nanoparticles was calculated about 11.13 nm diameter. The morphology of Fe3O4 nanoparticles was investigated by scanning electron microscope (SEM). SEM images of magnetite were found partially smooth. Spectra of EDX depicted the Fe, O and Cl elements in IONP. Magnetic properties were examined by vibrating sampling magnetometer (VSM). The blood toxicity was reported by blood contents in normal and diabetic blood samples for specified intervals using CBC technique. One sample test of variance of hemoglobin, erythrocytes, leucocytes and thrombocytes showed the significant difference (p > 0.05) among 0 hrs to 72 hrs for all normal and diabetic blood samples. This study concluded that presence of iron oxides nanoparticles of size 11.13 nm in human blood induces reactive oxygen species which cause the cell death. Hemoglobin was highly affected content of blood than erythrocytes, leucocytes by toxic effects of IONPs. Reduction of platelets among all content of blood groups posed another signature of this hematology study. Toxic spectrum of IONPs must be considered before the application of MRI contrast agents and drug delivery.

Author Biographies

Muhammad Javid ARSHAD, Department of Basic Sciences.University of Engineering & Technology, Taxila

Dr.M.Arshad Javid, Assistant Professor (Physics).

Department of Basic Sciences (Physics).University of Engineering & Technology, Taxila Pakistan 47050.

Saddique AMIR, Department of Basic Sciences (Physics), University of Engineering and Technology, Taxila

MS Student, Department of Basic Sciences (Physics),

Mehmood ZAHID, Department of Software Engineering, University of Engineering and Technology, Taxila, email;

Assistant Professor, Department of Software Engineering, University of Engineering and Technology, Taxila.

Husain FAYYAZ, Department of Physics, Bahudin Zakariya University, Multan, Pakistan, 60800

Assistant Professor (Statistics) , Department of Basic Sciences,

University of Engineering and Technology, Taxila.

Saeed Ahmad BUZDAR, Department of Physics, The Islamia University of Bahawalpur, Pakistan, 63100

Assistant Professor (Physics), Department of Physics,GC University, Faisalabad

Muhammad IMRAN, Department of Physics, Government College University, Faisalabad, Pakistan, 3800

Assistant Professor (Physics), Department of Physics, Bahhudin Zikriya University,

Published
2019-06-27
Section
BIOACTIVE MATERIALS