Synthesis, Characterization and Blood Based Toxic Effects of Superparamagnetic Nanoparticles

Abstract

The objective of this study is to the assess toxicity of superparamagnetic nanoparticles within normal and diabetic human blood groups using complete blood count (CBC). For this purpose, iron oxide nanoparticles (IONPs) were synthesized by co-precipitation method. Samples were characterized by XRD, SEM, EDS, VSM and CBC. XRD confirmed the cubic structure of Fe₃O₄ with Miller indices (2 0 0), (2 2 0), (3 1 1), (4 0 0), (4 4 0) and average size of magnetic nanoparticles was calculated about 11.13 nm diameter. The morphology of Fe₃O₄ nanoparticles was investigated by scanning electron microscope (SEM). SEM images of magnetite were found partially smooth. Spectra of EDX depicted the Fe, O and Cl elements in IONP. Magnetic properties were examined by vibrating sampling magnetometer (VSM). The blood toxicity was reported by blood contents in normal and diabetic blood samples for specified intervals using CBC technique. One sample test of variance of hemoglobin, erythrocytes, leucocytes and thrombocytes showed the significant difference (p > 0.05) among 0 hrs to 72 hrs for all normal and diabetic blood samples. This study concluded that presence of iron oxides nanoparticles of size 11.13 nm in human blood induces reactive oxygen species which cause the cell death. Hemoglobin was highly affected content of blood than erythrocytes, leucocytes by toxic effects of IONPs. Reduction of platelets among all content of blood groups posed another signature of this hematology study. Toxic spectrum of IONPs must be considered before the application of MRI contrast agents and drug delivery.