Application of PQQ-GDH Based Polymeric Layers in Design of Biosensors for Detection of Heavy Metals

Authors

  • I. Lapėnaitė, B. Kurtinaitienė, L. Pliuškys, V. Laurinavičius, I. Bachmatova, L. Marcinkevičienė, A. Ramanavičius∗ Department of Analytical and Environmental Chemistry, Vilnius University

Keywords:

heavy metals, cadmium, lead, biosensors, PQQ enzymes, glucose dehydrogenase, inhibition.

Abstract

A novel feature of biologically active polymer created by copolymerisation of glutaraldehyde (GA) with enzyme pyroloquinoline quinone dependent glucose dehydrogenase from Gluconobacter sp. 34 (PQQ-GDH) was its sensitivity to heavy metals. Examination of the influence of Cd2+ and Pb2+ ions on biologically active membranes was investigated. Both metal ions showed reversible inhibition of PQQ-GDH/GA copolymer. Inhibition effects of enzymatic activity of PQQ-dependent glucose dehydrogenase were applied in biosensors devoted for detection of Cd2+ and Pb2+ ions. Basic characteristics of biosensors were determined. Here presented biosensor can be applied for detection of Cd2+ and Pb2+ ions in concentration range of 15 ·10–5 mol/l.

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Published

2003-12-10

Issue

Vol. 9 No. 4 (2003)

Section

Articles

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