Achyranthes aspera-Mediated Silver Nanoparticle Synthesis for Enhanced Enrichment: Toxicological Studies on Eudrilus eugeniae Earthworms




Silver nanoparticles, Eudrilus eugeniae, toxicology, reproductive organ


This study utilizes gas chromatography mass spectrometry (GC-MS) to analyze phytochemicals in Achyranthes aspera, revealing over 57 phyto components in its methanol extracts. Major components include Neophytadiene, Stigmasterol, and Lupeol. The study then explores the formation of silver nanoparticles (AgNPs) using these extracts, observing a color change in the reaction mixture, indicating successful synthesis. UV/Vis spectra reveal absorption peaks at 343 and 411nm, indicative of surface plasmon resonance (SPR) in the AgNPs. Fourier-transform infrared spectroscopy (FTIR) highlights various functional groups present in the nanoparticles. X-ray diffraction (XRD) confirms the crystalline nature of the AgNPs, showing distinct peaks corresponding to crystallographic planes. SEM and AFM images display spherical AgNPs with cluster formations. The introduction of these plant-mediated AgNPs to earthworms results in significant growth compared to control groups. Silver content analysis in earthworms corroborates the effectiveness of the plant-mediated nanoparticles. Dissection analysis reveals enhanced growth in reproductive organs. Despite the benefits of nanomaterials, the study acknowledges potential toxicological concerns, emphasizing the increasing usage of silver nanoparticles and their potential impact on the environment. This research provides valuable insights into the synthesis, characterization, and biological effects of plant-mediated silver nanoparticles.

Author Biographies

Uma RAJALAKSHMI Thayalan, Rani Anna Government College for Women

PG and Research Department of Chemistry

ESAIVANI Chellaiah, Sarah Tucker College (Autonomous), Tirunelveli. Affiliated to M.S. University

PG Department of Zoology

ANANTHAKUMAR Thangaiya , Merit Arts and Science College

Department of Chemistry

Mariselvam Ramaiah, Saraswathi Institute of Lifesciences

Principal Scientist

Al OBAID sami, King Saud University

Department of Botany and Microbiology, College of Science

M. ALJAWDAH Hossam , King Saud University

Department of Zoology, College of Science